![]() To demonstrate the potential for internalization of the silk particles, both KELLY and THP-1-derived macrophages were exposed to fluorescently labelled silk particles for up to 24 h. When the cytotoxicity of the released doxorubicin was tested on KELLY neuroblastoma cells, significant cell death was observed. ![]() Doxorubicin loading in the silk particles was approximately 41 µg/mg sustained doxorubicin release occurred over 23 days. Binding and release of doxorubicin, a cytotoxic drug commonly used for cancer treatment, with the fabricated silk particles was evaluated. Lastly, decreasing the silk concentration from 12% to 2% resulted in a decrease in the median particle size from 5.6 to 2.8 μm and the PDI from 0.81 to 0.25, respectively. With a flow-focusing microfluidics device, lowering the silk flow rate from 0.80 to 0.06 mL/h resulted in a decrease in the median particle size from 6.8 to 3.0 μm and the PDI from 0.12 to 0.05, respectively. Utilizing a co-flow microfluidic device decreased the PDI of the silk particles as compared to an emulsion-based method (0.13 versus 0.65, respectively). Three main variables resulted in differences in particle size and size distribution, or polydispersity index (PDI). In this study, polydimethylsiloxane-based microfluidic devices were designed, fabricated, and characterized to produce silk particles through self-association of silk when exposed to PVA. Silk has been previously used to make particles using an emulsion-based method with poly(vinyl alcohol) (PVA). Bombyx mori silk fibroin has shown promise as a biocompatible biomaterial both in research and the clinic. Microparticles could be developed for direct injection of drug formulations into a diseased site, such as a tumor, allowing for drug retention and slow drug exposure over time through sustained release mechanisms. Step-9: When complete, the Product Activation will signify if the activation was successful.ġ) This process is not intended to float licenses between multiple users and there is a limit on the number of times a license can be transferred.Ģ) Without transferring the license if you install SolidWorks on the new system the following tab appears, then you need to contact your reseller.Polymeric particles are ideal drug delivery systems due to their cellular uptake-relevant size. Step-8: SOLIDWORKS will contact the Activation Server to activate the license on the computer. Ensure that valid email address is added to the Contact Information. Step-7: On the SOLIDWORKS Product Activation window, click Select All and then click Next. Step-6: Once installed, start SOLIDWORKS the Product Activation window will appear, click Next. Install SOLIDWORKS on the other or new computer using the same serial number as the previous installation. Step-5: Reactivate the SOLIDWORKS License Step-4: After transferring the license following screen appears, you have successfully transferred your license back to SolidWorks, ready to re-install on another computer. ![]() Step-3: SOLIDWORKS will contact the Activation Server to transfer the license off the computer. Ensure that valid email address is added to the Contact Information Step-2: After selecting Transfer Licenses from help, SolidWorks Product Activation window will appear, In that click Select All and then click Next. To do this open SOLIDWORKS and go to the SOLIDWORKS Help menu and click Transfer Licenses as Show in Image. Step-1: Transfer the existing SOLIDWORKS License.įirst, you need to transfer your SOLIDWORKS license back to the SOLIDWORKS Activation Server. However, you can transfer a license from one computer to another using the Transfer/Activate process. SOLIDWORKS Stand-alone licenses can only be active on 1 computer at a time.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |